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- We persistently found that the population of dividing astrocytes was significantly smaller on the facet by which N-CAM or one of the N-CAM-related molecules was infused than on the opposite aspect.
- Smith GM, Rutishauser U, Silver J, Miller RH. Maturation of astrocytes in vitro alters the extent and molecular basis of neurite outgrowth.
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As a control, a peptide of the same composition because the N-CAM decapeptide however of random sequence was infused on the contralateral aspect of the mind. We consistently discovered that the inhabitants of dividing astrocytes was significantly smaller on the aspect by which N-CAM or one of many N-CAM-related molecules was infused than on the alternative facet. The inhibition was best in the cortical lesion sites (approximately 50%) and was much less pronounced within the hippocampus (approximately 25%) and striatum (approximately 20%). Two weeks after the lesion, the cerebral cortical websites infused with N-CAM continued to exhibit a significantly smaller inhabitants of dividing astrocytes than the sites on the alternative facet. When N-CAM and fundamental fibroblast growth issue, which is known to stimulate astrocyte division in vitro, were coinfused into cortical lesion sites, astrocyte proliferation was nonetheless inhibited. These outcomes counsel the hypothesis that, by decreasing glial proliferation, N-CAM or its peptides could help create an environment that is extra appropriate for neuronal regeneration.
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After a penetrating lesion within the central nervous system, astrocytes enlarge, divide, and participate in creating an setting that adversely affects neuronal regeneration. We have just lately shown that the neural cell adhesion molecule (N-CAM) partially inhibits the division of early postnatal rat astrocytes in vitro. Animals had been lesioned in the cerebral cortex, hippocampus, or striatum with a Hamilton syringe and needle at defined stereotaxic positions. On one facet, the lesions had been concomitantly infused with N-CAM or with one of the N-CAM-related molecules.
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